Induction and enhancement of progressive motility in hamster caput epididymal spermatozoa
نویسندگان
چکیده
منابع مشابه
Motility induction in hamster spermatozoa from caput epididymidis: effects of forward motility protein (FMP) and calmodulin inhibitor.
The motility characteristics and the behaviour of caput spermatozoa were investigated. After dilution in B2 medium the majority of spermatozoa presented head-to-head agglutination and a twisted flagellum. Only a small population of these spermatozoa developed an anarchic motility pattern. The addition of crude bovine epididymal fluid forward motility protein (FMP) to the incubation medium suppr...
متن کاملDevelopment and initiation of sperm motility in the hamster epididymis.
Hamster spermatozoa were isolated from the caput, corpus and cauda epididymidis. They were observed in culture medium at 37 degrees C with a phase-contrast microscope and their motility recorded cinematographically. About 20 p. 100 of the caput epididymidis spermatozoa were motile and moved in a confined space with no forward progression. 30 p. 100 of the corpus epididymidis spermatozoa were mo...
متن کاملAdenine nucleotide mediation of fructolysis and motility in bovine epididymal spermatozoa.
Fructolysis is stimulated 3to 5-fold in bovine epididymal spermatozoa when the latter are preincubated with caffeine, theophylline, cyclic adenosine 3’, 5’-monophosphate (CAMP), and N6, 02’-dibutyryl adenosine 3’,5’-monophosphate’((Bt)2CAMP). No increase in fructolysis is observed if phosphodiesterase inhibitors or cyclic nucleotides are added to freshly washed cells or to preincubated cells ju...
متن کاملIn vivo fertilization after initiation of sperm motility in the hamster epididymis.
Hamster spermatozoa, collected from the caput and the cauda of the epididymis, are known to differ in their motility. They do not normally acquire fertilizing ability until they reach the proximal portion of the cauda epididymidis. The aim of the present investigation was to test the fertilizing capacity of spermatozoa from the caput epididymis after initiation. Sperm cells were incubated by ad...
متن کاملCharacterization of sulfhydryl proteins involved in the maintenance of flagellar straightness in hamster spermatozoa.
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing sperm sulfhydryl (SH) groups with diamide. In the present study, the authors examined the SH proteins involved in sperm flagellar bending using two-dimensional polyacrylamide gel electrophoresis and mon...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1986
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod35.4.1065